Conversion of Proline-14c to Collagen Trans-3-hydroxyproline-14c in the Chick Embryo.
نویسندگان
چکیده
trans-3-Hydroxy-nn-proline-3,4-3H was synthesized by reduction of N-carbobenzoxy-3-keto-DLproline methyl ester with NaBH4 (4). Uniformly labeled n-proline-r4C was obtained from New England Nuclear Corporation. Uniformly labeled 4-hydroxy-r.-prolineJ4C was generously donated to us by Dr. Chozo Mitoma. Seven-day-old chick embryos were obtained from the Duckworth Hatchery (Elkridge, Maryland) and incubated in a moist atmosphere at 37” for 1 to 2 days before use.
منابع مشابه
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 239 شماره
صفحات -
تاریخ انتشار 1964